Journal: PLOS One

Article Title: Xiao Qing Long Tang ameliorates neutrophil extracellular trap-dendritic cells-T helper 17 cell axis in Neutrophilic Asthma

doi: 10.1371/journal.pone.0336333

Figure Lengend Snippet: BMDCs were pretreated with control culture, Nets, OVA/LPS, OVA/LPS/Nets and then coculture with naïve CD4 + T lymphocytes. a. Representative flow cytometric analysis and comparisons of Th17 in each group. b Comparisons of concentrations of IL-17 in each group. c. Comparisons of concentrations of IL-6 in each group, d. Comparisons of concentrations of IL-23 in each group. e. Representative Western blot images and comparisons of p-p38/p38 MAPK, p-IKBα/IKBα, p65/p65, β-actin in each group. f. The images of immunohistochemical staining and comparisons for P-p38 MAPK, P-pIKBα, P-p65 expression in the CD11c+ positive cells of CON and OVA/LPS induced lung, (P-p38 in the lung were identified with DAPI (blue), P-p38 (red) and CD11c+ (green) by confocal microscopy, P-p65 in the lung were identified with DAPI (blue), P-p65 (red) and CD11c+ (green) by confocal microscopy, P-p65 in the lung were identified with DAPI (blue), P-pIKBα (red) and CD11c+ (green) by confocal microscopy). OVA/LPS/Nets-stimulated BMDCs were pretreated with control culture, OVA/LPS/Nets, p38 inhibitor (SB202190), OVA/LPS/Nets/SB202190, and then coculture with naïve CD4 + T lymphocytes, g. Representative Western blot images and comparisons of p-p38/p38 MAPK, p-IKBα/IKBα, p65/p65, β-actin. in each group, h. Comparisons of concentrations of IL-6 in each group, i. Comparisons of concentrations of IL-23 in each group. OVA/LPS/Nets-stimulated BMDCs were pretreated with control culture, OVA/LPS/Nets, NF-κB inhibitor (DHMEQ), OVA/LPS/Nets/DHMEQ, and then coculture with naïve CD4 + T lymphocytes, j. Representative Western blot images and comparisons of p-IKBα/IKBα, p65/p65, β-actin in each group, k. Comparisons of concentrations of IL-6 in each group, l. Comparisons of concentrations of IL-23 in each group. m. Representative flow cytometric analysis and comparisons of Th17 in CON, OLN, SB202190, OLNS group. n. Comparisons of concentrations of IL-17 in in CON, OLN, SB202190, OLNS group. o. Representative flow cytometric analysis and comparisons of Th17 in in CON, OLN, DHMEQ, OLND group. p Comparisons of concentrations of IL-17 in each group. (Data were means ± SEM (n = 3); * * P < 0.01) (CON: control group, Nets: Neutrophil extracellular traps group, OL: OVA/LPS group, OLN:OVA/LPS/Nets group, SB202190:a p38-MAPK specific inhibitor group, DHMEQ: a NF-κB-specific inhibitor group, OLNS:OVA/LPS/Nets/SB202190 group, OLND:OVA/LPS/Nets/DHMEQ group).

Article Snippet: To explore the signaling pathways involved in OVA/LPS/Nets-induced BMDCs in regulating Th17 cell differentiation, BMDCs were pre-treated with specific inhibitors: 10 μM p38-MAPK specific inhibitor SB202190(MCE, USA, HY-10295) and 5 μg/ml NF-κB-specific inhibitor DHMEQ (MCE, USA, HY-14645) 1 hour before being stimulated with OVA/LPS/Nets.

Techniques: Control, Western Blot, Immunohistochemical staining, Staining, Expressing, Confocal Microscopy

Journal: PLOS One

Article Title: Xiao Qing Long Tang ameliorates neutrophil extracellular trap-dendritic cells-T helper 17 cell axis in Neutrophilic Asthma

doi: 10.1371/journal.pone.0336333

Figure Lengend Snippet: BMDCs were pretreated with control culture (CON), OVA/LPS/Nets (OLN), XQLT, OVA/LPS/Nets/XQLT(OLNX) and then coculture with naïve CD4 + T lymphocytes. a. comparisons of concentrations of IL-6 in each group, b. comparisons of concentrations of IL-23 in each group, c. Representative flow cytometric analysis and comparisons of Th17 cells in each group, d. Comparisons of concentrations of IL-17 in each group, f. Representative Western blot images and comparisons of p-p38/p38 MAPK, p-IKBα/IKBα, p-p65/p65, β-actin in each group, f. The images of immunohistochemical staining and comparisons for P-p38 MAPK, P-pIKBα, P-p65 expression in the CD11c+ positive cells of lung, (P-p38 in the lung were identified with DAPI (blue), P-p38 (red) and CD11c+ (green) by confocal microscopy, P-p65 in the lung were identified with DAPI (blue), P-p65 (red) and CD11c+ (green) by confocal microscopy, P-p65 in the lung were identified with DAPI (blue), P-pIKBα (red) and CD11c+ (green) by confocal microscopy) (Data are means ± SEM (n = 3); ** P < 0.01) (CON: control group, XQLT: Xiao Qing Long Tang group, OLN: OVA/LPS/Nets group, OLNX:OVA/LPS/Nets/XQLT group).

Article Snippet: To explore the signaling pathways involved in OVA/LPS/Nets-induced BMDCs in regulating Th17 cell differentiation, BMDCs were pre-treated with specific inhibitors: 10 μM p38-MAPK specific inhibitor SB202190(MCE, USA, HY-10295) and 5 μg/ml NF-κB-specific inhibitor DHMEQ (MCE, USA, HY-14645) 1 hour before being stimulated with OVA/LPS/Nets.

Techniques: Control, Western Blot, Immunohistochemical staining, Staining, Expressing, Confocal Microscopy

Journal: STAR Protocols

Article Title: Protocol for functional screening of CFTR -targeted genetic therapies in patient-derived organoids using DETE CT OR deep-learning-based analysis

doi: 10.1016/j.xpro.2024.103593

Figure Lengend Snippet: Media to grow the colon human organoids (Organoid expansion medium)

Article Snippet: p38 MAPK inhibitor (p38i) (SB202190) , Selleckchem , S1077 , 100 mM , 0.5 mL , 10 mM.

Techniques: Concentration Assay